Urease. Part II. The Mechanism of the Zymolysis of Urea.
نویسنده
چکیده
Since this paper was submitted for publication, a paper has appeared on the mechanism of urease by Mack and Villars [1923]. In this paper the authors agree with the present writer in accepting the Werner formula for urea. They have detected and estimated cyanate in solutions of urea undergoing zymolysis, and also have shown that the cyanate rises to a maximum and falls again towards the end of the zymolysis. Their maximum values expressed as percentage cyanic acid agree closely with several maximum values obtained by the present writer (0.003 %). On the other hand, they arrive at the conclusion, by a process of elimination, that the transformation
منابع مشابه
The Significance of Cyanic Acid in the Urea- Tjrjxase System
The close chemical relationship between cyanic acid and urea suggests a corresponding biochemical relationship whenever urea is synthesized or decomposed in animal or vegetable tissues. For this reason the zymolysis of urea was studied by the writer (1) to see if cyanic acid could be detected among the products of the reaction, which would indicate that the enzyme decomposition of urea proceeds...
متن کاملبازدارندگی فلزات سنگین و حلال‌ها بر آنزیم اوره‌آز برای تولید آمونیاک در سنتز داروی ضدفشار خون نیفیدیپین
Introduction: Simultaneous development of technology is composed with environmental hazardous with heavy-metals and organic solvents and thus toxic catalysts/organic solvents must be replaced with biocatalysts and water. Urease is a hydrolase enzyme and urease-urea can be considered a safe source of toxic ammonia in synthesis of nifedipine. Nifedipine is an antihypertensive drug synthesized by ...
متن کاملUtilization of Urea and Urease Treated Rice Straw by Indigenous Growing Cattle
The experiment was conducted to study the effect of treatment of rice straw with urea and a urease containing midden soil on the chemical composition of treated rice straw, feed intake of the animals, nutrients digestibility, body weight gain, feed conversion efficiency and overall economy of feeding for a period of 105 days. Twelve indigenous growing cattle (live weight 130.00±1.67 kg) were se...
متن کاملA Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode
Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respo...
متن کاملویژگیهای سینتیکی آنزیم اوره‌آز در حضور رس‌های سپیولیت و ورمیکولیت
Urea is one of the most commonly used nitrogen fertilizers in agricultural lands. The fate of this fertilizer in soils is greatly related to the soil urease enzyme activity, while this enzyme commonly exists in the adsorbed state on the surface of soil colloids, and especially clay minerals. In order to examine the kinetic properties of free and adsorbed Urease on the surface of sepiolite and v...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The Biochemical journal
دوره 17 6 شماره
صفحات -
تاریخ انتشار 2005